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The score of PPIs in the subnetworks were also calculated.Briefly, all PPIs in the weighted network were ranked from large to small according to their weight coefficient and this was defined as the background set (E), whereas the subnetwork was defined as the objective set (S).Tissues samples from 32 severe and 40 mild NAFLD patients were evaluated to identify differentially expressed genes (DEGs) between the 2 groups, followed by analyses of Gene Ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomes pathways.Then, a weighted protein–protein interaction (PPI) network was constructed, and subnetworks and candidate genes were screened.The work cannot be changed in any way or used commercially without permission from the journal.

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indicated that the expression of certain metabolism-related genes was induced in severe NAFLD.

In this study, we thus used bioinformatics to identify candidate genes potentially causative of severe NAFLD.

Gene expression profile data GSE49541 were downloaded from the Gene Expression Omnibus database.

Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Website ().

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited.

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